mRNA features

• Exon

  • Initial, internal, terminal
    • Codon usage, preference
    • Control elements (e.g. splice enhancers)

• Intron

  • 5’ splice site (“GT”), branchpoint (lariat), 3’ splice site (“AG”)
  • Repeat elements

• Start codon (translation start site)

  • “Kozak” rule

• UTR (untranslated regions)

  • 5’ UTR
    • Translation regulatory elements
    • RNA binding sites
  • Initial, internal, terminal
    • Control elements (e.g. splice enhancers)
  • 3’ UTR
    • RNA binding sites (cis-acting elements)

• Stop codon

• Poly-adenylation signal and site

• RNA destabilization signal

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